组长简介
课题组长:吴志猛
电 话: 0510-85197582
Email: zwu@jiangnan.edu.cn
教育与工作经历:
江苏省“特聘教授”(2014)江南大学生物工程学院 教授 博士生导师 (2013至今)
新西兰皇家科学院工业研究院 研究科学家(2011-2013)
美国Wayne State University 化学系 博士后 (2008-2011)
美国Karmanos Cancer Institute 博士后 (2005-2008)
浙江大学 博士 (2002-2005)
主持/参与科研项目:
1:国家自然科学基金 糖基化复杂壁磷壁酸的全合成及其免疫活性研究(21472070,2015.1-2018.12)
2:江苏省特聘教授 糖类药物化学(2015.1-2017.12)
3:江苏省“双创计划”创新团队(2015.1-2017.12)
4:江苏省“六大人才高峰“项目
5:北京大学天然药物及仿生药物国家重点实验室开放课题
6:江苏省社发计划( BE2019632 )
7:无锡市卫生健康委科研项目
学术兼职:
担任中国化学会糖化学分会委员和江苏省免疫学会转化医学常务委员
受邀作为Chemical Communications, Journal of organic chemistry, Journal of Combinatorial chemistry, Organic & Biomolecular Chemistry, European Journal of Organic Chemistry, Current Organic Synthesis, European Journal of Medicinal Chemistry, Chemistry Letter, Australian journal of chemistry, Bioorganic & Medicinal Chemistry, Carbohydrate Research等期刊的审稿人
主要研究内容、目标及进展
从事糖化学与药物化学交叉的研究工作,致力于复杂寡糖、糖肽、糖脂及其它糖缀合物的合成新方法学研究 (包括化学合成和生物合成),并应用于化学生物学、药物化学、糖生物学、抗肿瘤和细菌疫苗糖免疫学等研究;另外课题组还致力于纳米抗体用于肿瘤免疫治疗、抗体偶联药物研发等;
研究队伍
课题组成员:
副研究员:周志昉
副研究员:施杰
博士后:洪皓飞
在读博、硕士研究生:
2016级博士研究生:林汉
2017级博士研究生:戴士杰
2018级博士研究生:周坤 沈鹏
2019级博士研究生:龚亮 李艳春.
2020级博士研究生:李丹 张自江
2018级硕士研究生: 解云天 刘金龙 梅国栋 陈文彦
2019级硕士研究生:陆仲锴 俞杭艳 张冉冉 鲍奕恺 刘治成 江敏
2020级硕士研究生:李霞 陆铭明 屈梦园 覃薪方 王征 赵洁 郑乐乐
本课题组期待每一位对我们课题感兴趣的高年级本科生,硕博士生和博士后的加盟;有意者请与我联系(生工A307室)。
代表性文章著作及专利
1. Haofei Hong, Chen Li, Liang Gong, Jinfeng Wang, Dan Li, Jie Shi, Zhifang Zhou, Zhaohui Huang* and Zhimeng Wu*. Universal endogenous antibody recruiting nanobodies capable of triggering immune effectors for targeted cancer immunotherapy. Chem. Sci., 2021, 12, 4623.
2. Shijie Dai, Haofei Hong, Kun Zhou, Kai Zhao, Yuntian Xie, Chen Li, Jie Shi, Zhifang Zhou, Lei Nie*, and Zhimeng Wu*. Exendin 4-hapten conjugate capable of binding with endogenous antibodies for peptide half-life extension and exerting long-acting hypoglycemic Activity. J. Med. Chem. 2021, DOI: 10.1021/acs.jmedchem.1c00032
3. Han Lin, Haofei Hong, Jinfeng Wang, Chen Li, Zhifang Zhou*, Zhimeng Wu*. Rhamnose modified bovine serum albumin as a carrier protein promotes the immune response against sTn antigen. Chem. Commun., 2020, 56, 13959. (Front Cover)
4. Shijie Dai, Shaozhong Liu, Chen Li, Zhifang Zhou, Zhimeng Wu*. Site-selective modification of exendin 4 with variable molecular weight dextrans by oxime-ligation chemistry for improving type 2 diabetic treatment. Carbohydr. Poly., 2020, 249, 116864.
5. Lipeng Feng, Jie Shi*, Haofei Hong, Zhifang Zhou, Zhimeng Wu*. GM3 trisaccharide biosynthesis and process optimization using engineered E. coli lysate and whole-cell catalysis. J. Carbohydr. Chem., 2020, 39 (5-6), 217-231
6. Dan Li, Surui Yao, Zhifang Zhou, Jie Shi, Zhaohui Huang∗, Zhimeng Wu∗. Hyaluronan decoration of milk exosomes directs tumor-specific delivery of doxorubicin. Carbohydr. Res.2020, 493, 108032.
7. Kai Zhao, Feng Tang, Wei Shi, Haofei Hong, Zhifang Zhou, Wei Huang, Zhimeng Wu*, One-step immobilization and purification of genetic engineering CBD fusion EndoS on cellulose for antibodies Fc-glycan remodeling, Bioorg. Chem., 2019, 91, 103114.
8. Haofei Hong, Zhifang Zhou, Kun Zhou, Shaozhong Liu, Zhongwu Guo* and Zhimeng Wu*, Site-specific C-terminal dinitrophenylation to reconstitute the antibody Fc functions for nanobodies, Chem. Sci., 2019, 10, 9331-9338.
9. Haofei Hong, Xinrui Zhao, ZhimengWu*. A two-stage glycine supplementation strategy enhances the extracellular expression of sortase A in Escherichia coli. Process Biochem., 2019, 76, 11.
10. Zhifang Zhou, Han Lin, Chen Li, Zhimeng Wu*, Recent progress of fully synthetic carbohydrate-based vaccine using TLR agonist as build-in adjuvant, Chin. Chem. Lett., 2018, 29, 19.
11. Min Yang, Haofei Hong, Shaozhong Liu, Xinrui Zhao and Zhimeng Wu*. Immobilization of staphylococcus aureus sortase A on chitosan particles and its applications in peptide-to-peptide ligation and peptide cyclization. Molecules 2018, 23, 192.
12. Xiaozhong Cheng, Haofei Hong, Zhifang Zhou, and Zhimeng Wu*. Enzymatic on-resin peptide cleavage and in Situ cyclization one-pot strategy for the synthesis of cyclopeptide and cyclotide. J. Org. Chem., 2018, 83, 14078.
13. Zhimeng Wu*, Shaozhong Liu, Xiaozhong Cheng, Xinrui Zhao, Haofei Hong. High yield synthesis of cyclic analogues of antibacterial peptides P-113 by Sortase A-mediated ligation and their conformation studies. Chin. Chem. Lett. 2017, 28, 553.
14. Xiaozhong Cheng, Tao Zhu, Haofei Hong, Zhifang Zhou and Zhimeng Wu*. Sortase A-mediated on-resin peptide cleavage and in situ ligation: an efficient one-pot strategy for the synthesis of functional peptides and proteins. Org. Chem. Front., 2017, 4, 2058.
授权专利:
1. 吴志猛,周志昉,赵恺;一种利用纤维素固定化CBD-EndoS融合酶的方法;中国专利;申请号:201810358946.3
2. 吴志猛,赵恺,洪皓飞,周志昉;一种高效表达糖苷内切酶Endo S或其突变体的方法;中国专利;申请号:201810900866.6
3. 吴志猛,周志昉,李晨,戴士杰,刘少中;带有羟胺基的艾塞那肽类似物及其应用;中国专利;授权日期2020.12.29;授权号ZL2018106131527
4. 吴志猛,赵鑫锐,洪皓飞,邓涛;一种构建大肠杆菌高效分泌表达转肽酶Sortase A的方法;中国专利;授权日期2020.07.07;授权号ZL2016104657810
5. 吴志猛,赵鑫锐,洪皓飞,邓涛;一种利用毕赤酵母高效分泌表达转肽酶Sortase A的方法;中国专利;授权日期2020.01.21;授权号ZL2016103086450
6. 吴志猛,程孝中,刘少中;一种酶法合成环肽的方法;中国专利;授权日期2019.08.20;授权号ZL2016108466225
7. 吴志猛,赵鑫锐,洪皓飞;一种高效分泌表达转肽酶Sortase A的方法; 中国专利;授权日期2018.04.06;授权号ZL2015104400328