欢迎来到糖化学与生物技术教育部重点实验室
糖苷酶与分子生物学研究室
  2017-05-18  

课题组组长:邬敏辰

出生日期: 19626

职称: 教授,博士生导师

Tel.: +86 510  85329081.

E-mail: biowmc@126.com

 

研究领域及方向

长期从事酶工程、分子生物学及生物催化等领域的科研工作

1. 糖苷水解酶及生物催化关键酶类的挖掘、分子改造及应用研究

2. 生物法制备功能性食品添加剂 (低聚木糖、甘露糖、苯乳酸、阿魏酸等) 及工艺开发

3. 生物法制备手性医药或精细化工中间体 (β-卤代醇、环氧化物、邻二醇等) 及工艺开发

 

教育、工作经历:

2012/9-至今 江南大学医学院,教授,博导

2008/6-2012/9 江南大学医药学院,教授,博导

2002/6-2008/6 江南大学医学院,副教授

1990.3~2000.10 江苏无锡启蒙生物高技术研究所,高级工程师,所长

1997/9–2001/3 复旦大学,分子生物学,博士

1987/9–1990/7 江南大学,粮油工程,硕士

1979/9–1983/7 东南大学,力学,学士

 

研科项目:

1. 基于理性合计的新型菜豆环氧化物水解酶定向改造,国家自然基金面上 (No.B060804)

2. 基于理性化设计的β-甘露聚糖酶分子定向改造,国家自然基金面上 (No.31271811)

3. 宇佐美曲霉木聚糖酶耐热/耐碱性的分子改造,国家自然基金青年 (No.31101229

4. 碱性脂肪酶热稳定性的生物信息学分析及定点突变,国家自然基金面上 (No.20776061)

5. 功能性低聚糖的酶法生产和生理功能研究,企业委托课题

6.  β-甘露聚糖酶产业化生产技术的开发与应用,杭州市科技发展计划项目

 

主要研究内容、目标及进展

多年来主要从事糖苷水解酶类的挖掘、分子改造及热稳定性机制研究。糖苷水解酶是一类重要的工业酶,但现有商品化的糖苷水解酶多数耐热性差,优良耐热糖苷水解酶在能源、食品、造纸和饲料等应用领域中具有广阔的市场需求。本课题组克隆和表达了曲霉来源的6种酸性中温木聚糖,3种酸性β-甘露聚糖酶 (其中AuMan26A100℃孵育10 min仍残留30%酶活性) 2种中温内切葡聚糖酶 (Biores Technol, 2008, 99(4): 831–838, J Agric Food Chem, 2012, 60(3): 765-773)。随后,借助多序列同源比对、同源建模和分子动力学模拟等手段进行理性设计,构建杂合木聚糖酶AEx11A (1) Topt80较野生型酶提高30 (Biotechnol Bioeng, 2013, 110: 1028-1038)杂合木聚糖酶AEXynM (2) Tm值为91.6reAusXyn11A高出34.0(Biotechnol Biofuels, 2014, 7(1): 3)杂合β-甘露聚糖酶AuMan5A-AfToptTm7576.6 °C野生型酶提高1012.1℃,催化效率也提高12.7 (Appl Microbiol Biotechnol, 2016, 100: 3989-3998)。本课题组拟进一步基于数据搜集与分析构建代表性糖苷水解酶文库,深入探究酶结构与热稳定性的关系,系统阐述酶的热稳定机制,并指导具有优良性质的中温酶的耐热性改造,获得具有自主知识产权的若干性质优良的耐热酶,从而推动耐热糖苷水解酶产业化生产的进程。


FIG


1 杂合木聚糖酶AEx11A三维结构的同源建模及分析 a: EvXyn11TSAoXyn11A N末端序列比对; b:活性中心E89-E180,盐桥K23-D24E35-R38,二硫键C5-C32; c:盐桥K23-D24的局部放大; d: 二硫键(C5-C32)的局部放大


1-1


2 杂合木聚糖酶AEXynM的空间结构分析 (a) AEXynM3-D结构;(b) AEXynM二硫键Cys5-Cys32(c) 疏水性作用(Pro9-Phe21-Trp22)和氢键His14-Phe17

 

 

学术兼职:

中国生物化学学会会员,中国免疫学会会员,中国微生物学会会员,中国发酵工业协会会员

 

研究队伍

教师:李剑芳 (教授邓超 (副教授) 丁磊 (副教授) 王克伟 (校聘副教授)

博士研究生:胡蝶,李闯,胡博淳

硕士研究生:叶慧华 吴芹唐诗涵 王瑞 石小玲 李雪晴 袁风娇阚婷婷 宗迅成 刘艳

近年来,指导学生11人次获 研究生国家奖学金

 

指导硕、博士生专业方向

发酵工程、生物化学与分子生物学、微生物与生化药学、制药工程

 

代表性文章、著作及专利

5年,发表国内国际期刊论文100余篇,其中SCI论文40余篇,累计IF80.5。近3年,申请国家发明专利20余篇。主编和参编著作5部。代表性期刊论文如下:

2017

[1] Li XQ, Wu Q, Hu D, Wang R, Liu Y, Wu MC, Li JF. Improving the temperature characteristics and catalytic efficiency of a mesophilic xylanase from Aspergillus oryzae, AoXyn11A, by iterative mutagenesis based on in silico design. AMB express, 2017 (Receive).(IF=2.167)

[2] Li JF#, Wang CJ#, Hu D, Yuan FJ. Li XQ, Tang SH, Wu MC*. Engineering a family 27 carbohydrate-binding module into an Aspergillus usamii β-mannanase to perfect its enzymatic properties. Journal of Bioscience and Bioengineering. 2017 123(3):294–299 (IF=1.964)

2016

[1] Dong YH#, Li JF#, Hu D, Yin X, Wang CJ, Tang SH, Wu MC*. Replacing a piece of loop-structure in the substrate-binding groove of Aspergillus usamiiβ-mannanase, AuMan5A, to improve its enzymatic properties by rational design. Appl Microbiol Biotechnol, 2016, 100(9): 3989-3998. (IF=3.376)

[2] Yu T#, Li JF#, Zhu LJ, Hu D, Deng C, Cai YT, Wu MC*. Reduction of m-chlorophenacyl chloride coupled with regeneration of NADPH by recombinant Escherichia coli cells co-expressing both carbonyl reductase and glucose 1-dehydrogenase. Ann Microbiol, 2016, 66(1): 343-350.(IF= 1.232)

[3] Hu D#, Wang R#, Shi XL, Ye HH, Wu Q, Wu MC*. Jian-Jun Chu*. Kinetic resolution of racemic styrene oxide at a high concentration by recombinant Aspergillus usamii epoxide hydrolase in an n-hexanol/buffer biphasic system. J Biotechnol, 2016, 236: 152–158.   (IF= 2.667)

[4] Ye HH#, Hu D#, Shi XL, Wu MC*, Deng C, Li JF*. Directed modification of a novel epoxide hydrolase from Phaseolus vulgaris to improve its enantioconvergence towards styrene epoxides. Catalysis Communications, 2016, 87: 32-35. (IF= 3.389)

[5] Hu D #, Li JF#, Wu Q, Zang J, Cheng JQ, Wu MC*. Improved Temperature Characteristics of an Aspergillus oryzae GHF11 Xylanase, by In Silico Design and Site-Directed Mutagenesis. Biotechnology and Bioprocess Engineering. 2016, 21: 704-711. (IF= 1.211)

2015

[1] Hu D#, Tang CD#, Yang B, Liu JC, Yu T, Deng C, Wu MC*. Expression of a novel epoxide hydrolase of Aspergillus usamii E001 in Escherichia coli and its performance in resolution of racemic styrene oxide. J Ind Microbiol Biotechnol, 2015, 42(5): 671-680. (IF=2.439)

[2] Yin X#, Hu D#, Li JF, He Y, Zhu TD, Wu MC*. Contribution of disulfide bridges to the thermostability of a type A feruloyl esterase from Aspergillus usamii. PLoS ONE, 2015, 10(5): e0126864. (IF=3.234)

[3] Hu D#, Ye HH#, Wu MC*, Feng F, Zhu LJ, Yin X, Li JF*. Chemoenzymatic preparation of (S)-p-nitrostyrene oxide from p-nitrophenacyl bromide by recombinant Escherichia colicells expressing a novel halohydrin dehalogenase. Catalysis Communications, 2015, 69(1): 72-75. (IF=3.699)

[4] Yin X#, Li JF#, Wang CJ, Hu D, Wu Q, Gu Y, Wu MC*. Improvement in the thermostability of a type A feruloyl esterase, AuFaeA, from Aspergillus usamii by iterative saturation mutagenesis. Appl Microbiol Biotechnol, 2015, 99(23): 10047–10056. (IF=3.337)

2014

[1] Zhang HM#, Li JF#, Wang JQ, Yang YJ, Wu MC*. Determinants for the improved thermostability of a mesophilic family 11 xylanase predicted by computational methods. Biotechnol Biofuels, 2014, 7(1):3. (IF=6.221)

[2] Tan ZB#, Li JF#, Li XT, Gu Y, Wu MC*, Wu J, Wang JQ. A unique mono- and diacylglycerol lipase from Penicillium cyclopium: heterologous expression, biochemical characterization and molecular basis for its substrate selectivity. Plos One, 2014, 9(7): e102040.(IF=3.534)

[3] Li JF, Wei XH, Tang CD, Wang JQ, Zhao M, Pang QF, Wu MC*. Directed modification of the Aspergillus usamiiβ-mannanase to improve its substrate affinity by in silico design and site-directed mutagenesis. J Ind Microbiol Biotechnol, 2014, 41(4): 693-700. (IF=2.505)

[4] Zhang HM, Wang JQ, Wu MC*, Gao SJ, Li JF, Yang YJ. Optimized expression, purification and characterization of a family 11 xylanase (AuXyn11A) from Aspergillus usamii E001 in Pichia pastoris. J Sci Food Agric, 2014, 94(4): 699-706. (IF=1.879)

[5] Li JF#, Zhang HM#, Wu MC*, Wang CJ, Dong YH, Zhu LJ, Zhang P. Expression and characterization of hyperthermotolerant xylanases, SyXyn11P and SyXyn11E, in Pichia pastoris and Escherichia coli. Appl Biochem Biotechnol, 2014, 172(7): 3476-3487. (IF=1.687)

[6] Tan ZB#, Li JF#, Wu MC*, Wang JQ. Enhancing the thermostability of a cold-active lipase from Penicillium cyclopium by In silico design of a disulfide bridge. Appl Biochem Biotechnol, 2014, 173(7): 1752-1764. (IF=1.687)

[7] Wang JQ#, Tan ZB#, Wu MC*, Li JF, Wu J. Improving the thermostability of a mesophilic family 10 xylanase, AuXyn10A, from Aspergillus usamii by in silico design. J Ind Microbiol Biotechnol, 2014, 41(8): 1217-1225.(IF=2.505)

[9] Tang CD#, Guo J#, Li JF, Wei XH, Hu D, Gao SJ, Yin X, Wu MC*. Enhancing expression level of an acidophilic β-mannanase in Pichia pastoris by double vector system. Ann Microbiol, 2014, 64: 561–569.(IF=1.039)

[10] Zeng Y#, Yin X#, Wu MC*, Yu T, Feng F, Zhu TD, Pang QF. Expression of a novel feruloyl esterase from Aspergillus oryzae in Pichia pastoris with esterification activity. J Mol Catal B-Enzym, 2014, 110: 140–146. (IF=2.745)

[11] Tan ZB#, Tang CD#, Wu MC*, He Y, Hu D, Wang JQ. Exploration of disulfide bridge and N-Glycosylation contributing to high thermostability of a hybrid xylanase. Protein & Peptide Letters, 2014, 21(7): 657-662. (IF=1.735)

[12] Luo QL, Wu J*, Wu MC. Enhanced acetoin production by Bacillus amyloliquefaciens through improved acetoin tolerance. Process Biochem, 2014, 49(8): 1223-1230. (IF=2.516)

[13] Wang WL#*, Huang C#, Gao LX, Tang CL, Wang JQ, Wu MC, Sheng L, Chen HJ, Nan FJ, Li JY, Li J*, Feng BN*. Synthesis and biological evaluation of novel bis-aromatic amides as novel PTP1B inhibitors. Bioorg Med Chem Lett, 2014, 24(8): 1889-1894.(IF=2.420)

 

奖项与荣誉

中国石油和化学工业协会科技进步奖二等奖

珠海市科学技术奖二等奖

无锡市科学技术奖二等奖

无锡市具有突出贡献的中青年专家

江南大学青蓝工程优秀青年骨干教师等荣誉称号

技术支持:信息化建设与管理中心
校内备案号:JW备170140

地址:江苏省无锡市蠡湖大道1800号
邮编:214122
联系电话:0510-85912032
服务邮箱:netser@jiangnan.edu.cn